OPEN POSITIONS

Postdoctoral fellow to work on AML

We are currently looking for a motivated candidate to jointly apply to the MARIE SKŁODOWSKA-CURIE INDIVIDUAL FELLOWSHIP program of the EU in the next call (deadline 12th September 2018). Information can be found in the following link

The candidate will focus on investigating the contribution of the bone marrow microenvironment to acute myeloid leukemia (AML) progression and maintenance of therapy-resistant leukemia initiating cells. We will employ advanced mouse models to dissect the role of different stromal cell types in the pathology of AML.

Candidates should hold a PhD, and background/high-level knowledge in flow cytometry and confocal microscopy. Experience in RNAseq technologies and analysis will be highly valued. We are looking for a person with a strong drive, a proven capacity to work independently but always keen on collaborating with the rest of the lab team. Candidates interested please submit ASAP a motivation letter (email) and CV to: Cesar.NombelaArrieta@usz.ch

MECHANO-REGULATION OF CELLULAR INTERPLAY BETWEEN MESENCHYMAL, HEMATOPOIETIC AND VASCULAR SYSTEMS IN THE BONE MARROW MICROENVIRONMENT

Master thesis Project (9-12 months)

Host labs: Ralph Müller (ETH)-César Nombela-Arrieta (UZH, USZ)


Short abstract: Bone marrow tissues are essential for sustained production of blood cellular components, the continuous remodeling of bone and efficient immune responses. This project aims to investigate the mechano-regulation of mesenchymal, hematopoietic and vascular systems in the bone marrow microenvironment.


Keywords: 3D confocal microscopy, in vivo micro-CT, bone marrow microenvironment, stem cells, niche, mechano-regulation


Description: Bone marrow tissues are essential for the sustained production of blood cellular components, the continuous remodeling of bone surfaces and the mounting of efficient immune responses. The sophisticated regulation of these biological processes is achieved through the complex cellular interplay established between bone marrow mesenchymal (bone-forming), hematopoietic and vascular systems present. A key unresolved question is how mechanical forces acting on bone shape the tissue microarchitectural organization, the cellular composition and the function of underlying bone marrow tissues. To address these questions, global approaches are needed that enable the registration of mechanical effects in bone morphology, the visualization of subsequent vascular remodeling and changes in extracellular matrix structure and the analyses of cellular content in single bones. The aim of this project is to combine powerful multidimensional imaging and cytometry technologies developed in the laboratory of Prof. Ralph Müller (ETH) and Prof. César Nombela-Arrieta (UZH, USZ) to provide such comprehensive data. The student will work in integrating imaging data from bone surfaces obtained using dynamic in vivo micro-CT, with 3D fluorescence confocal microscopy of bone and marrow tissues at subcellular resolution, and advanced flow cytometric techniques. Ultimately, the student will perform an exploratory analysis on how mechanical loading modifies the microenvironment and hematopoietic stem cell regulation in murine bone marrow.  Students with strong interest in state of the art imaging technologies and computational analyses are encouraged to apply.


Goal: The goal is to establish computational methods to correlate data obtained by multiple imaging approaches using registration techniques previously developed in the Müller lab. By means of these techniques we aim to quantify the effects of mechanical loading in the mesenchymal, hematopoietic and vascular components of the marrow.


Contact Details: Prof. Dr. Ralph Müller, ram@ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller; Prof. Dr. César Nombela Arrieta, Cesar.NombelaArrieta@usz.ch, Department of Experimental Hematology, University Hospital Zurich and University of Zurich

PhD student positions open!

We are currently looking for 2 candidates interested in starting a PhD in our group. Our current projects  focus in investigating  the functional interplay of stromal bone marrow cells and hematopoiesis in stead-state as well as in murine models of disease. Work will involve the use of new mouse models, flow cytometry, single cell RNA-seq and state of the art 3D-quantitative imaging techniques.  We are looking for students with a strong interest in the basic biology of the regulation of hematopoiesis and hematopoietic stem cells, but also a very clear drive towards the use of new technologies, including advanced microscopy and single-cell platforms. Candidates are expected to apply and be accepted to the Life Science Graduate School of the University of Zurich and ETH (https://www.lifescience-graduateschool.uzh.ch/en.html). If interested, please send a motivation letter (email) and your CV to: Cesar.NombelaArrieta@usz.ch